Semax is a synthetic heptapeptide analog of the adrenocorticotropic hormone (ACTH) fragment 4-10, with the modified sequence Met-Glu-His-Phe-Pro-Gly-Pro. The C-terminal Pro-Gly-Pro extension confers enhanced stability against enzymatic degradation compared to the native ACTH fragment. Semax has a molecular weight of approximately 813.93 Da and has been extensively studied in neuroscience research applications, particularly in models of neuroprotection and cognitive function.
In neuronal cell culture models, Semax has been shown to upregulate brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) expression. Quantitative PCR analysis of treated cortical neuron cultures demonstrates significant increases in BDNF mRNA within hours of compound exposure. This neurotrophic modulation has positioned Semax as a valuable research tool for investigating neuroplasticity mechanisms and neurotrophic signaling cascades.
Preclinical neuroprotection models have utilized Semax extensively. In oxygen-glucose deprivation (OGD) models using hippocampal slice cultures, Semax pretreatment has been associated with reduced lactate dehydrogenase (LDH) release, a marker of cellular injury. Gene expression profiling of Semax-treated neuronal cultures has identified modulation of over 100 genes involved in vascular function, inflammation, and neuronal survival pathways. Specifically, downregulation of pro-inflammatory cytokines including IL-1β and TNF-α has been observed in microglial cell models.
Additional research applications include investigation of melanocortin receptor (MC3R/MC4R) signaling, as Semax retains affinity for these receptors. In immune cell models, Semax has demonstrated immunomodulatory properties, including modulation of cytokine profiles in peripheral blood mononuclear cell (PBMC) cultures. Cognitive function research in preclinical models has explored Semax in the context of cholinergic and dopaminergic pathway modulation.
Erosyn Labs provides Semax as a lyophilized powder at ≥98% purity verified by HPLC. Each lot includes mass spectrometry identity confirmation and a full Certificate of Analysis. Reconstitution in sterile water is recommended. The lyophilized compound should be stored at -20°C. Reconstituted solutions are stable at 2–8°C for up to 21 days. Standard peptide handling protocols apply, including aseptic technique and avoidance of repeated freeze-thaw cycles.
For research and laboratory use only. Not for human consumption.
